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Important new tool for single-molecule localization microscopy
March 2016. Single-localization microscopy is a wonderful tool to unravel the mysteries of molecular and cellular architecture at nanoscopic level and is more and more widely used by microscopists. Many scientists tend to think that almost any kind of biological structure under 10 nm could be resolved with this approach. However, this is not the case and typically used image performance measures such as resolution and localization precision do not fully predict whether is possible for a given structure of interest. The new software SuReSim (short for "Super-Resolution Simulaton"), developed by the group of Thomas Kuner at Heidelberg University together with Mike Heilemann at the Goethe University Frankfurt with support from CEF, provides microscopists now with a tool for exploring a large range of labeling and imaging parameters in advance of biological experiments, thus saving valuable time and resources. SuReSim helps not only to predict whether a biological structure of interest can be resolved with single-moleculare locatlization microscopy, but also provides a tool for efficient experimental design and for avoiding under- or overinterpretation of imaging data. A detailed descritption of the software along with examples was published in the journal Nature Methods. Further information ...
Contact:
Mike Heilemann
Institute of Physical and Theoretical Chemistry
Goethe University Frankfurt
Max von Laue Straße 9
60438 Frankfurt, Germany
Heilemann@chemie.uni-frankfurt.de
Reference:
Venkataramani V, Herrmannsdorfer F, Heilemann M, Kuner T (2016) SuReSim: simulating localization microscopy experiments from ground truth models. Nature Methods, published online 29 February 2016. Link