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Super-chelators for advanced protein labeling in living cells

February 2018. Live-cell labeling, super-resolution microscopy, single-molecule applications, protein localization or chemically induced assembly are emerging approaches, which require specific and very small interaction pairs. The minimal disturbance of protein function is essential to derive unbiased insights into cellular processes. Scientists at Goethe University Frankfurt report a new class of hexavalent N-nitrilotriacetic acid (hexaNTA) chelators, displaying the highest affinity and stability of all NTA-based small interaction pairs described so far. Coupled to bright organic fluorophores with fine-tuned photo-physical properties, these super-chelator probes were delivered into human cell cultures by chemically gated nanopores. These super-chelators permit kinetic profiling, multiplexed labeling of His6 and His12-tagged proteins as well as single-molecule based super-resolution imaging.

 

Contact:
Robert Tampé, Institute of Biochemistry, Riedberg Campus, Goethe University Frankfurt/M., Germany, Tel.: +49 (0)69 798-29475, tampe@em.uni-frankfurt.de

 

Publication:
Gatterdam K, Joest EF, Dietz MS, Heilemann M, Tampé R (2018) Super-chelators for advanced protein labeling in living cells. Angew Chem Int Ed: published online 20 February 2018. http://dx.doi.org/10.1002/anie.201800827